ELISA data for serum IL-6 after LPS or SAL treatment
Data analysis of serum protein expression of IL-6 after treatment with saline (SAL) or 4500 EU/g lipopolysaccharide (LPS) for 18 hours. Data are pg/mL.
Samples
## Group TRT n
## 1 CON SAL 37
## 2 CON LPS 52
## 3 MIA SAL 45
## 4 MIA LPS 68Figure 1g
Linear Mixed Effect Model MIA vs CON
## CON:SAL CON:LPS MIA:SAL MIA:LPS
## # mice 37 52 45 68
## # litters 26 34 28 43## normality of log2 transformed data## Shapiro Test for Normality
## CON:SAL 0.001
## CON:LPS 0.000
## MIA:SAL 0.002
## MIA:LPS 0.000## Linear mixed model fit by REML. t-tests use Satterthwaite's method [
## lmerModLmerTest]
## Formula: log2(Serum.IL.6 + 1) ~ Group * TRT + (1 | Litter)
## Data: MvC
##
## REML criterion at convergence: 846.7
##
## Scaled residuals:
## Min 1Q Median 3Q Max
## -1.5590 -0.7317 -0.1818 0.5063 2.5996
##
## Random effects:
## Groups Name Variance Std.Dev.
## Litter (Intercept) 0.7935 0.8908
## Residual 3.4080 1.8461
## Number of obs: 200, groups: Litter, 86
##
## Fixed effects:
## Estimate Std. Error df t value Pr(>|t|)
## (Intercept) 2.2030 0.3620 156.2463 6.086 8.59e-09 ***
## GroupMIA 0.3506 0.4858 150.8619 0.722 0.472
## TRTLPS 7.2278 0.4272 170.7738 16.919 < 2e-16 ***
## GroupMIA:TRTLPS -0.5081 0.5726 176.4439 -0.887 0.376
## ---
## Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
##
## Correlation of Fixed Effects:
## (Intr) GrpMIA TRTLPS
## GroupMIA -0.745
## TRTLPS -0.717 0.535
## GMIA:TRTLPS 0.535 -0.721 -0.746Reproducibility info
## [1] "2021-11-02 11:23:03 EDT"## ─ Session info ───────────────────────────────────────────────────────────────
## setting value
## version R version 4.0.3 (2020-10-10)
## os macOS Big Sur 10.16
## system x86_64, darwin17.0
## ui X11
## language (EN)
## collate en_US.UTF-8
## ctype en_US.UTF-8
## tz America/New_York
## date 2021-11-02
##
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##
## [1] /Library/Frameworks/R.framework/Versions/4.0/Resources/library