Results & Scripts

RNA sequencing analysis of microglia in Fig 1 & Ext Data Fig 1-3:

• MACS-MG Adult LPS Response
• FACS-MG Blunting
• Cell Type Heatmap_MACS
• Volcanos
• Cell Type Heatmap_FACS
• Regional MG Blunting

MIA IL-6 serum resonse to LPS Fig 1g: IL-6

MIA microglia morphology in Fig 1 & Ext Data Fig 4:

• CD68
• Sholl
• Density

Single Cell RNA sequencing in Fig 1, 4, & Ext Data 5: script

Microglia In Vitro Activation in Fig 2 and Ext Data Fig 10: In Vitro

RNA sequencing of neonatal microglia in Fig 2: P4 MG

Disease enrichment of DEGs Fig 2g:

• ADHD
• ASD
• BP
• MDD
• SZ
• multiple comparison correction

ATAC Sequencing in Fig 3, Ext Data Fig 6:

• ATAC
• Validation by Gene Expression
• PECA Network Analysis
• CUT&RUN

Rescue of Microglia Blunting with Prenatal Microglia Replacement (Fig 4-5, Ext Data Fig 7-8):

• Prenatal Microglia Ablation and Replacement
• 3 Hour Maternal Serum
• Amelioration of Microglia Blunting
• Rescue PCA
• Rescue volcanos

Rescue of microglia bluting in protein expression Fig 4-5: Protein Rescue

MSN Electrophysiology Fig. 5, Ext Data 9:

• MIA MSN phenotype
• Rescue of MSN
• Spine Analysis

No Effect of Sex by qPCR Ext Data Fig 10: qPCR